Surface modification of polyacrylonitrile with nitrile hydratase and amidase from Agrobacterium tumefaciens

被引：15

作者：

Fischer-Colbrie, Gudrun

Herrmann, Melanie

Heumann, Sonja

Puolakka, Arja

Wirth, Amina

Cavaco-Paulo, Artur

Guebitz, Georg M.

机构：

[1] Graz Univ Technol, Dept Environm Biotechnol, A-8010 Graz, Austria

[2] Res Ctr Appl Biocatalysis, Graz, Austria

[3] Tampere Univ Technol, FIN-33101 Tampere, Finland

[4] Univ Appl Sci NW Switzerland, Basel, Switzerland

[5] Univ Minho, Dept Text Engn, Guimaraes, Portugal

来源：

BIOCATALYSIS AND BIOTRANSFORMATION | 2006年 / 24卷 / 06期

关键词：

Agrobacterium tumefaciens; nitrile hydratase; polyacrylonitrile;

D O I：

10.1080/10242420601033977

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new strain of Agrobacterium tumefaciens (BST05) was found to grow on polyacrylonitrile (PAN; C-13 labelled) converting the polymer to polyacrylic acid as shown by solid state NMR. When cultivated in a medium containing acetonitrile the bacterium produced nitrile hydratase and amidase activity. Activity recovery after lyophilisation and enzyme stability was significantly enhanced in the presence of 5% sorbitol leading to half life times of 12, 72 and 154 days at 25 degrees C, 4 degrees C and -20 degrees C. The enzymes were able to convert 1.1% of the nitrile groups of PAN-powder to the corresponding acids. PAN fabrics were mainly converted to the amides as shown by an 80% increase of the O/C ratio in ESCA analysis. These data were confirmed by cationic dyeing and FTIR-ATR analysis.

引用

页码：419 / 425

页数：7

共 21 条

[11] BENCH-SCALE PRODUCTION OF ACRYLAMIDE USING THE RESTING CELLS OF BREVIBACTERIUM SP CH2 IN A FED-BATCH REACTOR [J].