Sensitive amperometric immunosensing using polypyrrolepropylic acid films for biomolecule immobilization

被引:76
作者
Dong, Hua
Li, Chang Ming
Chen, Wei
Zhou, Qin
Zeng, Zhao Xian
Luong, John H. T.
机构
[1] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore 639798, Singapore
[2] Watson Pharma Inc, Phoenix, AZ 85043 USA
[3] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada
关键词
D O I
10.1021/ac060657o
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An electrochemical immunosensor was constructed using an electropolymerized pyrrolepropylic acid (PPA) film with high porosity and hydrophilicity. A high density of carboxyl groups of PPA was used to covalently attach protein probes, leading to significantly improved detection sensitivity compared with conventional entrapment methods. As a model, anti-mouse IgG was covalently immobilized or entrapped in the PPA film and used in a sandwich-type alkaline phosphatase-catalyzing amperometric immunoassay with p-aminophenyl phosphate as the substrate. With covalent binding, the detection limit for IgG in PBS buffer, pH 7.4, was 100 pg/mL with a dynamic range of 5 orders of magnitude. The covalent bonding mode in the carbonate-bicarbonate buffer, pH 9.6, further brought down the detection limit to 20 pg/mL with remarkable selectivity.
引用
收藏
页码:7424 / 7431
页数:8
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