Dynamics of asexual transmission of a mitochondrial plasmid in Cryphonectria parasitica

被引:14
作者
Baidyaroy, D
Glynn, JM
Bertrand, H [1 ]
机构
[1] Michigan State Univ, Dept Microbiol, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Bot & Plant Pathol, E Lansing, MI 48824 USA
关键词
heterokaryon incompatibility; het; senescence; mitochondrial hypovirulence;
D O I
10.1007/s002940050527
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
In the chestnut blight fungus Cryphonectria parasitica, as in most fungi, little is known about the efficiency of the asexual transmission of optional mitochondrial plasmids, vertically through conidia, and horizontally through hyphal anastomoses. In this paper, we show that pCRY1, a circular mitochondrial plasmid, is transmitted vertically with 100%-efficiency through conidia. Moreover, the plasmid is transmitted horizontally through hyphal contact from donor strains to vegetatively compatible and most incompatible strains. An allelic difference between the donor and recipient strain, at only one of the five nuclear incompatibility genes that were tested strongly inhibited, but did not absolutely prevent, the transfer of pCRY1 through hyphal fusions. In contrast, allelic differences in any one or several of the other four heterokaryon-compatibility loci suppressed the transmission of the plasmid only partially or not at all. The plasmid was also transmitted among incompatible strains by protoplast fusion without the concomitant transfer of mitochondrial DNA (mtDNA). A comparison of plasmid-bearing with plasmid-free isogenic strains revealed that pCRY1 significantly diminishes the pathogenic potency of some strains of the fungus, but does not affect the virulence of others. Collectively, the observations indicate that the introduction of deleterious mitochondrial genetic elements into natural populations may be a means for managing fungal pathogens.
引用
收藏
页码:257 / 267
页数:11
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