Characterization of the dehydro-guanidinohydantoin oxidation product of guanine in a dinucleotide

被引：29

作者：

Chworos, A ^{[1
]}

Seguy, C ^{[1
]}

Pratviel, G ^{[1
]}

Meunier, B ^{[1
]}

机构：

[1] CNRS, Chim Coordinat Lab, F-31077 Toulouse 04, France

来源：

CHEMICAL RESEARCH IN TOXICOLOGY | 2002年 / 15卷 / 12期

关键词：

D O I：

10.1021/tx0200717

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The study of the biological consequences of oxidative damage to DNA requires the characterization of DNA lesions at a molecular level. The oxidation of guanine in the dinucleoside monophosphate, d(GpT), by a metal-oxo porphyrin produces a dehydroguanidinohydantoin derivative. The(1)H NMR spectrum of this oxidized guanine derivative is reported. The structure was confirmed by the chemical transformation of the dehydroguanidinohydantoin derivative into a linear oxaluric acid derivative upon hydrolysis at ambient temperature. The proposed mechanism of formation of the dehydro-guanidinohydantoin derivative is in agreement with the labeling experiments performed with (H2O)-O-18.

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页码：1643 / 1651

页数：9

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