Sequence analysis of the rfb loci, encoding proteins involved in the biosynthesis of the Salmonella enterica O17 and O18 antigens:: Serogroup-specific identification by PCR

被引:20
作者
Fitzgerald, Collette [1 ]
Gheesling, Linda [1 ]
Collins, Marcus [1 ]
Fields, Patricia I. [1 ]
机构
[1] Ctr Dis Control & Prevent, NCID, Div Bacterial & Mycot Dis, Food & Diarrheal Dis Branch, Atlanta, GA 30333 USA
关键词
D O I
10.1128/AEM.01046-06
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We report sequencing of the O antigen encoded by the rfb gene cluster of Salmonella enterica serotype Jangwani (O17) and Salmonella serotype Cerro (O18). We developed serogroup O17- and O18-specific PCR assays based on rfb gene targets and found them to be sensitive and specific for rapid identification of Salmonella serogroups O17 and O18.
引用
收藏
页码:7949 / 7953
页数:5
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