Analysis of CcpA mutations defective in carbon catabolite repression in Bacillus megaterium

被引：10

作者：

Kraus, A ^{[1
]}

Hillen, W ^{[1
]}

机构：

[1] UNIV ERLANGEN NURNBERG,LEHRSTUHL MIKROBIOL,INST MIKROBIOL BIOCHEM & GENET,D-91058 ERLANGEN,GERMANY

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 153卷 / 01期

关键词：

D O I：

10.1016/S0378-1097(97)00264-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Five mutations in ccpA of Bacillus megaterium with impaired functions were analysed for carbon catabolite repression. The phenotypes support the hypothesis that CcpA assumes a PurR/LacI fold. The completely inactive mutants CcpA119GE and CcpA326am cause alterations which are incompatible with that fold. A mutation with reduced activity, CcpA81GE, affects a site that would be partially surface exposed and may interfere with structure formation or cofactor binding. A mutation in the putative hinge alpha-helix, CcpA52AE, is negative transdominant over wild-type ccpA. The mutant CcpA38am is inactive, although reduced amounts of wild-type size protein are produced.

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页码：221 / 226

页数：6

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