Polymerase Chain Reaction Based Scaffold Preparation for the Production of Thin, Branched DNA Origami Nanostructures of Arbitrary Sizes

被引：108

作者：

Pound, Elisabeth ^{[1
]}

Ashton, Jeffrey R. ^{[1
]}

Becerril, Hector A. ^{[1
]}

Woolley, Adam T. ^{[1
]}

机构：

[1] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA

来源：

NANO LETTERS | 2009年 / 9卷 / 12期

基金：

美国国家科学基金会;

关键词：

SINGLE-STRANDED-DNA; FOLDING DNA; TETRAHEDRON; OCTAHEDRON; SHAPES;

D O I：

10.1021/nl902535q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Designs for DNA origami have previously been limited by the size of the available single-stranded genomes for scaffolds. Here we present a straightforward method for the production of scaffold strands having various lengths, using polymerase chain reaction amplification followed by strand separation via streptavidin-coated magnetic beads. We have applied this approach in assembling several distinct DNA nanostructures that have thin (similar to 10 nm) features and branching points, making them potentially useful templates for nanowires in complex electronic circuitry.

引用

页码：4302 / 4305

页数：4

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