ICP-MS as a novel detection system for quantitative element-tagged immunoassay of hidden peanut allergens in foods

被引:47
作者
Careri, Maria [1 ]
Elviri, Lisa [1 ]
Mangia, Alessandro [1 ]
Mucchino, Claudio [1 ]
机构
[1] Univ Parma, Dipartimento Chim Gen & Inorgan, I-43100 Parma, Italy
关键词
ICP; MS detection; ELISA; food allergens;
D O I
10.1007/s00216-006-1091-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A novel ICP-MS-based ELISA immunoassay via element-tagged determination was devised for quantitative analysis of hidden allergens in food. The method was able to detect low amounts of peanuts (down to approximately 2 mg peanuts kg(-1) cereal-based matrix) by using a europium-tagged antibody. Selectivity was proved by the lack of detectable cross-reaction with a number of protein-rich raw materials.
引用
收藏
页码:1851 / 1854
页数:4
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