Oncogenic transformation by β-catenin:: deletion analysis and characterization of selected target genes

被引：28

作者：

Aoki, M ^{[1
]}

Sobek, V

Maslyar, DJ

Hecht, A

Vogt, PK

机构：

[1] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA

[2] Univ Freiburg, Inst Mol Med & Zellforsch, Freiburg, Germany

来源：

ONCOGENE | 2002年 / 21卷 / 46期

关键词：

beta-catenin; LEF/TCF; target genes; oncogenic transformation;

D O I：

10.1038/sj.onc.1205796

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic analysis of beta-catenin-induced oncogenic transformation in chicken embryo fibroblasts (CEF) revealed the following prerequisites for oncogenicity: (1) removal of the N terminal phosphorylation sites targeted by glycogen synthase kinase 3beta (GSK3beta), (2) retention of the N terminal transactivation domain, and (3) retention of the armadillo repeats. The C terminal transactivation domain played an ancillary role in the transformation of CEF. There was a rough correlation between the transforming activity of various beta-catenin constructs and their transactivation of the TOPFLASH reporter. Expression levels of the candidate target genes of beta-catenin-LEF, cyclin D1 and myc were not correlated with each other or with the transforming activity of beta-catenin constructs. A new target gene, coding for inositol hexakisphosphate kinase 2 (IP6K2) was identified. Its expression showed concordance with the transforming activity of beta-catenin constructs.

引用

页码：6983 / 6991

页数：9

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