Genetic typing and HIV-1 diagnosis by using 96 capillary array electrophoresis and ultraviolet absorption detection

被引：18

作者：

Gong, XY

Yeung, ES ^{[1
]}

机构：

[1] Iowa State Univ, Ames Lab, USDOE, Ames, IA 50011 USA

[2] Iowa State Univ, Dept Chem, Ames, IA 50011 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 2000年 / 741卷 / 01期

基金：

美国国家卫生研究院;

关键词：

genetic typing;

D O I：

10.1016/S0378-4347(00)00165-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Current high-throughput approaches to the analysis of PCR products are based primarily on electrophoretic separation and laser-excited fluorescence detection. We show that capillary array electrophoresis can be applied to HIV-1 diagnosis and D1S80 VNTR genetic typing based simply on UV absorption detection. The additive contribution of each base pair to the total absorption signal provides adequate detection sensitivity for analyzing most PCR products. Not only is the use of specialized and potentially toxic fluorescent labels eliminated, but also the complexity and cost of the instrumentation are greatly reduced. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：15 / 21

页数：7

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