Inhibitory effect of heparin on serotonin-induced hyperplasia and hypertrophy of smooth muscle cells

被引:11
作者
Lee, SL
Wang, WW
Joseph, PM
Hales, CA
Fanburg, BL
机构
[1] TUFTS UNIV,SCH MED,NEW ENGLAND MED CTR,DIV PULM,DEPT MED,BOSTON,MA 02111
[2] TUFTS UNIV,SCH MED,NEW ENGLAND MED CTR,DIV CRIT CARE,DEPT MED,BOSTON,MA 02111
[3] HARVARD UNIV,MASSACHUSETTS GEN HOSP,SCH MED,BOSTON,MA
关键词
D O I
10.1165/ajrcmb.17.1.2727
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Serotonin (5-HT) produces both hyperplastic and hypertrophic effects on smooth muscle cell (SMC) in culture. Heparin is known to inhibit serum-induced hyperplasia of SMC but has not been previously tested on the stimulatory effect of 5-HT on SMC. Our present data show that at 24 h heparin inhibited by 50% the stimulation of H-3-thymidine incorporation into bovine pulmonary artery SMC and at 7 days totally reversed both cellular proliferation and enlargement of SMC produced by 1 mu M 5-HT. Heparin failed to alp ter 5-HT uptake by SMC, but inhibited the stimulation of tyrosine phosphorylation of GTPase-activating protein, a proposed intermediate in the 5-HT stimulatory process. Thus heparin inhibits both hyperplastic and hypertrophic effects of 5-HT on SMC, perhaps through the inhibition of a phosphorylated intermediate protein.
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页码:78 / 83
页数:6
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