Conversion of AFLP fragments tightly linked to SCMV resistance genes Scmv1 and Scmv2 into simple PCR-based markers

被引：27

作者：

Dussle, CM ^{[1
]}

Quint, M ^{[1
]}

Xu, ML ^{[1
]}

Melchinger, AE ^{[1
]}

Lübberstedt, T ^{[1
]}

机构：

[1] Univ Hohenheim, Dept Plant Breeding Seed Sci & Populat Genet, D-70593 Stuttgart, Germany

来源：

THEORETICAL AND APPLIED GENETICS | 2002年 / 105卷 / 08期

关键词：

AFLP; CAPS; indel marker; maize; marker conversion; sugarcane mosaic virus (SCMV);

D O I：

10.1007/s00122-002-0964-7

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In a previous study, bulked segregant analysis with amplified fragment length polymorphisms (AFLPs) identified several markers closely linked to the sugarcane mosaic virus resistance genes Scmv1 on chromosome 6 and Scmv2 on chromosome 3. Six AFLP markers (E33M61-2, E33M52, E38M51, E82M57, E84M59 and E93M53) were located on chromosome 3 and two markers (E33M61-1 and E35M62-1) on chromosome 6. Our objective in the present study was to sequence the respective AFLP bands in order to convert these dominant markers into more simple and reliable polymerase chain reaction (PCR)-based sequence-tagged site markers. Six AFLP markers resulted either in complete identical sequences between the six inbreds investigated in this study or revealed single nucleotide polymorphisms within the inbred lines and were, therefore, not converted. One dominant AFLP marker (E35M62-1) was converted into an insertion/deletion (indel) marker and a second AFLP marker (E33M61-2) into a cleaved amplified polymorphic sequence marker. Mapping of both converted PCR-based markers confirmed their localization to the same chromosome region (E33M61-2 on chromosome 3; E35M62-1 on chromosome 6) as the original AFLP markers. Thus, these markers will be useful for marker-assisted selection and facilitate map-based cloning of SCMV resistance genes.

引用

页码：1190 / 1195

页数：6

共 31 条

[1] Development of a SCAR (sequence characterised amplified region) marker for molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus L. [J].

Barret, P ;

Delourme, R ;

Foisset, N ;

Renard, M .

THEORETICAL AND APPLIED GENETICS, 1998, 97 (5-6) :828-833

[2]

BORCHARDT DC, 2000, EUCARPIA QUANTITATIV, P213

[3] Conversion of an AFLP fragment linked to the carrot Y2 locus to a simple, codominant, PCR-based marker form [J].

Bradeen, JM ;

Simon, PW .

THEORETICAL AND APPLIED GENETICS, 1998, 97 (5-6) :960-967

[4] MOLECULAR-GENETICS OF GROWTH AND DEVELOPMENT IN POPULUS .3. A GENETIC-LINKAGE MAP OF A HYBRID POPLAR COMPOSED OF RFLP, STS, AND RAPD MARKERS [J].

BRADSHAW, HD ;

VILLAR, M ;

WATSON, BD ;

OTTO, KG ;

STEWART, S ;

STETTLER, RF .

THEORETICAL AND APPLIED GENETICS, 1994, 89 (2-3) :167-178

[5] Comparison of the genetic maps of Brassica napus and Brassica oleracea [J].

Cheung, WY ;

Champagne, G ;

Hubert, N ;

Landry, BS .

THEORETICAL AND APPLIED GENETICS, 1997, 94 (05) :569-582

[6] A potato hypersensitive resistance gene against potato virus X maps to a resistance gene cluster on chromosome 5 [J].

DeJong, W ;

Forsyth, A ;

Leister, D ;

Gebhardt, C ;

Baulcombe, DC .

THEORETICAL AND APPLIED GENETICS, 1997, 95 (1-2) :246-252

[7] Molecular mapping and gene action of Scm1 and Scm2, two major QTL contributing to SCMV resistance in maize [J].

Dussle, CM ;

Melchinger, AE ;

Kuntze, L ;

Stork, A ;

Lübberstedt, T .

PLANT BREEDING, 2000, 119 (04) :299-303

[8]

FUCHS E, 1995, Z PFLANZENK PFLANZEN, V102, P44

[9] DNA sequence evidence for the segmental allotetraploid origin of maize [J].

Gaut, BS ;

Doebley, JF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (13) :6809-6814

[10]

Green P, 1990, CRI MAP VERSION 2 4

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