Conversion of AFLP fragments tightly linked to SCMV resistance genes Scmv1 and Scmv2 into simple PCR-based markers

被引：27

作者：

Dussle, CM ^{[1
]}

Quint, M ^{[1
]}

Xu, ML ^{[1
]}

Melchinger, AE ^{[1
]}

Lübberstedt, T ^{[1
]}

机构：

[1] Univ Hohenheim, Dept Plant Breeding Seed Sci & Populat Genet, D-70593 Stuttgart, Germany

来源：

THEORETICAL AND APPLIED GENETICS | 2002年 / 105卷 / 08期

关键词：

AFLP; CAPS; indel marker; maize; marker conversion; sugarcane mosaic virus (SCMV);

D O I：

10.1007/s00122-002-0964-7

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In a previous study, bulked segregant analysis with amplified fragment length polymorphisms (AFLPs) identified several markers closely linked to the sugarcane mosaic virus resistance genes Scmv1 on chromosome 6 and Scmv2 on chromosome 3. Six AFLP markers (E33M61-2, E33M52, E38M51, E82M57, E84M59 and E93M53) were located on chromosome 3 and two markers (E33M61-1 and E35M62-1) on chromosome 6. Our objective in the present study was to sequence the respective AFLP bands in order to convert these dominant markers into more simple and reliable polymerase chain reaction (PCR)-based sequence-tagged site markers. Six AFLP markers resulted either in complete identical sequences between the six inbreds investigated in this study or revealed single nucleotide polymorphisms within the inbred lines and were, therefore, not converted. One dominant AFLP marker (E35M62-1) was converted into an insertion/deletion (indel) marker and a second AFLP marker (E33M61-2) into a cleaved amplified polymorphic sequence marker. Mapping of both converted PCR-based markers confirmed their localization to the same chromosome region (E33M61-2 on chromosome 3; E35M62-1 on chromosome 6) as the original AFLP markers. Thus, these markers will be useful for marker-assisted selection and facilitate map-based cloning of SCMV resistance genes.

引用

页码：1190 / 1195

页数：6

共 31 条

[11] Confirmation of QTL associated with common bacterial blight resistance in four different genetic backgrounds in common bean [J].

Jung, G ;

Skroch, PW ;

Nienhuis, J ;

Coyne, DP ;

Arnaud-Santana, E ;

Ariyarathne, HM ;

Marita, JM .

CROP SCIENCE, 1999, 39 (05) :1448-1455

[12]

Kosambi D. D., 1944, ANN EUGENICS, V12, P172

[13] Resistance of early-maturing European maize germplasm to sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV) [J].

Kuntze, L ;

Fuchs, E ;

Gruntzig, M ;

Schulz, B ;

Klein, D ;

Melchinger, AE .

PLANT BREEDING, 1997, 116 (05) :499-501

[14]

LANDER E S, 1987, Genomics, V1, P174, DOI 10.1016/0888-7543(87)90010-3

[15]

LUBBERSTEDT T, 1999, P 15 EUCARPIA 1998 G, P241

[16] Genomic organization of disease and insect resistance genes in maize [J].

McMullen, MD ;

Simcox, KD .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1995, 8 (06) :811-815

[17] Conversion of AFLP bands into high-throughput DNA markers [J].

Meksem, K ;

Ruben, E ;

Hyten, D ;

Triwitayakorn, K ;

Lightfoot, DA .

MOLECULAR GENETICS AND GENOMICS, 2001, 265 (02) :207-214

[18] Genetic basis of resistance to sugarcane mosaic virus in European maize germplasm [J].

Melchinger, AE ;

Kuntze, L ;

Gumber, RK ;

Lübberstedt, T ;

Fuchs, E .

THEORETICAL AND APPLIED GENETICS, 1998, 96 (08) :1151-1161

[19] The major resistance gene cluster in lettuce is highly duplicated and spans several megabases [J].

Meyers, BC ;

Chin, DB ;

Shen, KA ;

Sivaramakrishnan, S ;

Lavelle, DO ;

Zhang, Z ;

Michelmore, RW .

PLANT CELL, 1998, 10 (11) :1817-1832

[20] Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection [J].

Negi, MS ;

Devic, M ;

Delseny, M ;

Lakshmikumaran, M .

THEORETICAL AND APPLIED GENETICS, 2000, 101 (1-2) :146-152

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