Fluorescence visualization of newly synthesized proteins in mammalian cells

被引：244

作者：

Beatty, Kimberly E.

Liu, Julie C.

Xie, Fang

Dieterich, Daniela C.

Schuman, Erin M.

Wang, Qian

Tirrell, David A.

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA

[3] CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USA

[4] CALTECH, Div Biol, Pasadena, CA 91125 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 44期

关键词：

alkynes; azides; fluorescent probes; protein modifications; proteomics;

D O I：

10.1002/anie.200602114

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Playing tag: Noncanonical amino acid tagging enables the selective fluorescent visualization of newly synthesized proteins in mammalian cells (see the picture). Susceptibility to tagging is determined by the spatial and temporal character of the protein synthesis, thus providing a complement to methods which identify relevant members of the proteome. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：7364 / 7367

页数：4

共 38 条

[21] Rostovtsev V. V., 2002, ANGEW CHEM, V114, P2708, DOI DOI 10.1002/1521-3757(20020715)114:14<2708::AID-ANGE2708>3.0.CO
[22] 2-0
[23] Rostovtsev VV, 2002, ANGEW CHEM INT EDIT, V41, P2596, DOI 10.1002/1521-3773(20020715)41:14<2596::AID-ANIE2596>3.0.CO
[24] 2-4
[25] A fluorogenic 1,3-dipolar cycloaddition reaction of 3-azidocoumarins and acetylenes
Sivakumar, K
Xie, F
Cash, BM
Long, S
Barnhill, HN
Wang, Q
[J]. ORGANIC LETTERS, 2004, 6 (24) : 4603 - 4606
[26] Profiling enzyme activities in vivo using click chemistry methods
Speers, AE
Cravatt, BF
[J]. CHEMISTRY & BIOLOGY, 2004, 11 (04): : 535 - 546
[27] Compartmentalized synthesis and degradation of proteins in neurons
Steward, O
Schuman, EM
[J]. NEURON, 2003, 40 (02) : 347 - 359
[28] Peptidotriazoles on solid phase: [1,2,3]-triazoles by regiospecific copper(I)-catalyzed 1,3-dipolar cycloadditions of terminal alkynes to azides
Tornoe, CW
Christensen, C
Meldal, M
[J]. JOURNAL OF ORGANIC CHEMISTRY, 2002, 67 (09) : 3057 - 3064
[29] Selective staudinger modification of proteins containing p-azidophenylalanine
Tsao, ML
Tian, F
Schultz, PG
[J]. CHEMBIOCHEM, 2005, 6 (12) : 2147 - 2149
[30] The green fluorescent protein
Tsien, RY
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 : 509 - 544

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