Evaluation of different detection methods of biofilm formation in Staphylococcus aureus

被引：147

作者：

Knobloch, JKM ^{[1
]}

Horstkotte, MA ^{[1
]}

Rohde, H ^{[1
]}

Mack, D ^{[1
]}

机构：

[1] Univ Hamburg, Hosp Eppendorf, Inst Med Mikrobiol & Immunol, D-20246 Hamburg, Germany

来源：

MEDICAL MICROBIOLOGY AND IMMUNOLOGY | 2002年 / 191卷 / 02期

关键词：

Staphylococcus aureus; biofilm; Congo red;

D O I：

10.1007/s00430-002-0124-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The icaADBC gene locus of Staphylococcus aureus and its polysaccharide intercellular adhesin (PIA/ PNSG) were recently identified, but biofilm formation has rarely been detected in vitro. In this study we evaluated a tissue culture plate (TCP) assay and a tube test, as well as Congo red agar, using the two basic media trypticase soy broth (TSB) and brain heart infusion (BHI) broth with different sugar supplements for detection of biofilm formation in 128 ica-positive S. aureus isolates. Of the S. aureus strains, 57.1% displayed a biofilm-positive phenotype under optimized conditions in the TCP test. The tube test correlated well with the TCP test for strongly biofilm-producing strains, whereas weak producers were not safely discriminated from biofilm-negative strains. Screening on Congo red agar displayed a strong correlation with the TCP and the tube test for only 3.8%, and is therefore not recommended for investigation of biofilm formation in S. aureus.

引用

页码：101 / 106

页数：6

共 47 条

[1] PREDOMINANCE OF 2 NEWLY DESCRIBED CAPSULAR POLYSACCHARIDE TYPES AMONG CLINICAL ISOLATES OF STAPHYLOCOCCUS-AUREUS [J].

ARBEIT, RD ;

KARAKAWA, WW ;

VANN, WF ;

ROBBINS, JB .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1984, 2 (02) :85-91

[2] Presence of icaA and icaD genes and slime production in a collection of staphylococcal strains from catheter-associated infections [J].

Arciola, CR ;

Baldassarri, L ;

Montanaro, L .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (06) :2151-2156

[3] A rapid PCR method for the detection of slime-producing strains of Staphylococcus epidermidis and S-aureus in periprosthesis infections [J].

Arciola, CR ;

Collamati, S ;

Donati, E ;

Montanaro, L .

DIAGNOSTIC MOLECULAR PATHOLOGY, 2001, 10 (02) :130-137

[4] PHASE VARIATION OF SLIME PRODUCTION IN STAPHYLOCOCCUS-AUREUS - IMPLICATIONS IN COLONIZATION AND VIRULENCE [J].

BASELGA, R ;

ALBIZU, I ;

DELACRUZ, M ;

DELCACHO, E ;

BARBERAN, M ;

AMORENA, B .

INFECTION AND IMMUNITY, 1993, 61 (11) :4857-4862

[5] CHEMICAL AND BIOLOGICAL PROPERTIES OF EXTRACELLULAR SLIME PRODUCED BY STAPHYLOCOCCUS-AUREUS GROWN IN HIGH-CARBOHYDRATE, HIGH-SALT MEDIUM [J].

BROCK, JH ;

REITER, B .

INFECTION AND IMMUNITY, 1976, 13 (03) :653-660

[6] MORPHOLOGICAL EXAMINATION OF THE GLYCOCALYCES OF STAPHYLOCOCCUS-AUREUS STRAINS WILEY AND SMITH [J].

CAPUTY, GG ;

COSTERTON, JW .

INFECTION AND IMMUNITY, 1982, 36 (02) :759-767

[7] ADHERENCE OF SLIME-PRODUCING STRAINS OF STAPHYLOCOCCUS-EPIDERMIDIS TO SMOOTH SURFACES [J].

CHRISTENSEN, GD ;

SIMPSON, WA ;

BISNO, AL ;

BEACHEY, EH .

INFECTION AND IMMUNITY, 1982, 37 (01) :318-326

[8] ADHERENCE OF COAGULASE-NEGATIVE STAPHYLOCOCCI TO PLASTIC TISSUE-CULTURE PLATES - A QUANTITATIVE MODEL FOR THE ADHERENCE OF STAPHYLOCOCCI TO MEDICAL DEVICES [J].

CHRISTENSEN, GD ;

SIMPSON, WA ;

YOUNGER, JJ ;

BADDOUR, LM ;

BARRETT, FF ;

MELTON, DM ;

BEACHEY, EH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 22 (06) :996-1006

[9] The intercellular adhesion (ica) locus is present in Staphylococcus aureus and is required for biofilm formation [J].

Cramton, SE ;

Gerke, C ;

Schnell, NF ;

Nichols, WW ;

Götz, F .

INFECTION AND IMMUNITY, 1999, 67 (10) :5427-5433

[10] In vitro methods to study staphylococcal biofilm formation [J].

Cramton, SE ;

Gerke, C ;

Götz, F .

MICROBIAL GROWTH IN BIOFILMS, PT A: DEVELOPMENTAL AND MOLECULAR BIOLOGICAL ASPECTS, 2001, 336 :239-255

← 1 2 3 4 5 →