Effects of Endotoxin and Catecholamines on Hepatic Mitochondrial Respiration

被引：14

作者：

Porta, Francesca ^{[1
,2
]}

Bracht, Hendrik ^{[1
,2
]}

Weikert, Christian ^{[1
,2
]}

Beck, Mario ^{[1
,2
]}

Takala, Jukka ^{[1
,2
]}

Brandt, Sebastian ^{[2
,3
]}

Hiltebrand, Luzius B. ^{[2
,3
]}

Jakob, Stephan M. ^{[1
,2
]}

机构：

[1] Univ Hosp Bern, Dept Intens Care Med, Inselspital, CH-3010 Bern, Switzerland

[2] Univ Bern, CH-3010 Bern, Switzerland

[3] Univ Hosp Bern, Dept Anesthesiol & Pain Therapy, Inselspital, CH-3010 Bern, Switzerland

来源：

INFLAMMATION | 2009年 / 32卷 / 05期

关键词：

endotoxin; liver; mitochondria; catecholamines; SKELETAL-MUSCLE; LIVER MITOCHONDRIA; HEART-MITOCHONDRIA; RAT HEPATOCYTES; DOPAMINE; METABOLISM; TOXICITY; KIDNEY; DAMAGE; BRAIN;

D O I：

10.1007/s10753-009-9138-y

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Catecholamines are frequently used in sepsis, but their interaction with mitochondrial function is controversial. We incubated isolated native and endotoxin-exposed swine liver mitochondria with either dopamine, dobutamine, noradrenaline or placebo for 1 h. Mitochondrial State 3 and 4 respiration and their ratio (RCR) were determined for respiratory chain complexes I, II and IV. All catecholamines impaired glutamate-dependent RCR (p = 0.046), predominantly in native mitochondria. Endotoxin incubation alone induced a decrease in glutamate-dependent RCR compared to control samples (p = 0.002). We conclude that catecholamines and endotoxin impair the efficiency of mitochondrial complex I respiration in vitro.

引用

页码：315 / 321

页数：7

共 27 条

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