DNA end-binding specificity of human Rad50/Mre11 is influenced by ATP

被引:51
作者
de Jager, M
Wyman, C
van Gent, DC
Kanaar, R
机构
[1] Erasmus MC, Dept Cell Biol & Genet, NL-3000 DR Rotterdam, Netherlands
[2] Erasmus MC Daniel, Dept Radiat Oncol, Rotterdam, Netherlands
关键词
D O I
10.1093/nar/gkf574
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Rad50, Mre11 and Nbs1 complex is involved in many essential chromosomal organization processes dealing with DNA ends, including two major pathways of DNA double-strand break repair, homologous recombination and non-homologous end joining. Previous data on the structure of the human Rad50 and Mre11 (R/M) complex suggest that a common role for the protein complex in these processes is to provide a physical link between DNA ends such that they can be processed in an organized and coordinated manner. Here we describe the DNA binding properties of the R/M complex. The complex bound to both single-stranded and double-stranded DNA. Scanning force microscopy analysis of DNA binding by R/M showed the requirement for an end to form oligomeric R/M complexes, which could then migrate or transfer away from the end. The R/M complex had a lower preference for DNA substrates with 3'-overhangs compared with blunt ends or 5'-overhangs. Interestingly, ATP binding, but not hydrolysis, increased the preference of R/M binding to DNA substrates with 3'-overhangs relative to substrates with blunt ends and 5'-overhangs.
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收藏
页码:4425 / 4431
页数:7
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