Cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo

被引:219
作者
Oyama, Tomomi
Nagai, Toshio
Wada, Hiroshi
Naito, Atsuhiko Thomas
Matsuura, Katsuhisa
Iwanaga, Koji
Takahashi, Toshinao
Goto, Motohiro
Mikami, Yoko
Yasuda, Noritaka
Akazawa, Hiroshi
Uezumi, Akiyoshi
Takeda, Shin'ichi
Komuro, Issei [1 ]
机构
[1] Chiba Univ, Grad Sch Med, Dept Cardiovasc Sci & Med, Chuo Ku, Chiba 2608670, Japan
[2] Fujita Hlth Univ, Inst Comprehens Med Sci, Div Therapies Intractable Dis, Aichi 4701192, Japan
[3] Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Dept Mol Therapy, Tokyo 1878502, Japan
关键词
D O I
10.1083/jcb.200603014
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Side population (SP) cells, which can be identified by their ability to exclude Hoechst 33342 dye, are one of the candidates for somatic stem cells. Although bone marrow SP cells are known to be long-term repopulating hematopoietic stem cells, there is little information about the characteristics of cardiac SP cells (CSPs). When cultured CSPs from neonatal rat hearts were treated with oxytocin or trichostatin A, some CSPs expressed cardiac-specific genes and proteins and showed spontaneous beating. When green fluorescent protein-positive CSPs were intravenously infused into adult rats, many more ( similar to 12- fold) CSPs were migrated and homed in injured heart than in normal heart. CSPs in injured heart differentiated into cardiomyocytes, endothelial cells, or smooth muscle cells (4.4%, 6.7%, and 29% of total CSP-derived cells, respectively). These results suggest that CSPs are intrinsic cardiac stem cells and involved in the regeneration of diseased hearts.
引用
收藏
页码:329 / 341
页数:13
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