Three-Dimensional Stimulated Emission Depletion Microscopy of Nitrogen-Vacancy Centers in Diamond Using Continuous-Wave Light

被引：128

作者：

Han, Kyu Young ^{[1
,2
]}

Willig, Katrin I. ^{[1
]}

Rittweger, Eva ^{[1
]}

Jelezko, Fedor ^{[3
]}

Eggeling, Christian ^{[1
]}

Hell, Stefan W. ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37077 Gottingen, Germany

[2] Seoul Natl Univ, Dept Chem, Seoul 151747, South Korea

[3] Univ Stuttgart, Inst Phys, D-70550 Stuttgart, Germany

来源：

NANO LETTERS | 2009年 / 9卷 / 09期

关键词：

SINGLE DEFECT CENTERS; FLUORESCENCE MICROSCOPY; STED MICROSCOPY; COLOR-CENTERS; RESOLUTION; BREAKING; NANODIAMONDS; REVEALS; BARRIER;

D O I：

10.1021/nl901597v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Charged nitrogen-vacancy (NV) color centers in diamond are excellent luminescence sources for far-field fluorescence nanoscopy by stimulated emission depletion (STED). Here we show that these photostable color centers can be visualized by STED using simple continuous-wave or high repetition pulsed lasers (76 MHz) at wavelengths >700 nm for STED. Furthermore, we show that NV centers can be imaged in three dimensions (M) inside the diamond crystal and present single-photon signatures of single color centers recorded in high density samples, demonstrating a new recording scheme for STED and related far-field nanoscopy approaches. Finally, we exemplify the potential of using nanodiamonds containing NV centers as luminescence tags in STED microscopy. Our results offer new experimental avenues in nanooptics, nanotechnology, and the life sciences.

引用

页码：3323 / 3329

页数：7

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