A novel class of self-sufficient cytochrome P450 monooxygenases in prokaryotes

被引：85

作者：

De Mot, R ^{[1
]}

Parret, AHA ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Ctr Microbial & Plant Genet, B-3001 Heverlee, Belgium

来源：

TRENDS IN MICROBIOLOGY | 2002年 / 10卷 / 11期

关键词：

D O I：

10.1016/S0966-842X(02)02458-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Bacillus cytochrome P450 BM3 integrates an entire P450 system in one polypeptide and represents a convenient prokaryotic model for microsomal P450s. This self-sufficient class 11 P450 is also present in actinomycetes and fungi. By genome analysis we have identified additional homologues in the pathogenic species Bacillus anthracis and Bacillus cereus, and in Ralstonia metallidurans. This analysis also revealed a novel class of putative self-sufficient P450s, P450 PFOR, comprising a class I P450 that is related to Rhodococcus erythropolis CYP116, and a phthalate family oxygenase reductase (PFOR) module. P450 PFOR genes are found in a Rhodococcus strain, three pathogenic Burkholderia species and in the R. metallidurans strain that possesses a P450 BM3 homologue. Co-evolution of P450 and reductase domains is apparent in both types of self-sufficient enzymes. The new class of P450 enzymes is of potential interest for various biotechnological applications.

引用

页码：502 / 508

页数：7

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