Three-dimensional reconstruction with contrast transfer function correction from energy-filtered cryoelectron micrographs: Procedure and application to the 70S Escherichia coli ribosome

被引:125
作者
Zhu, J
Penczek, PA
Schroder, R
Frank, J
机构
[1] NEW YORK STATE DEPT HLTH, WADSWORTH CTR LABS & RES, ALBANY, NY 12201 USA
[2] SUNY ALBANY, SCH PUBL HLTH, DEPT BIOMED SCI, ALBANY, NY 12201 USA
[3] MAX PLANCK INST MED RES, DEPT BIOPHYS, D-69120 HEIDELBERG, GERMANY
关键词
D O I
10.1006/jsbi.1997.3845
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cryoelectron microscopy provides the means of studying macromolecules in their native state. However, the contrast transfer function (CTF) makes the images and the three-dimensional (3D) maps derived from them difficult to interpret. We developed methods to determine the CTF from experimental data and to obtain a CTF-corrected 3D reconstruction. The CTF correction and 3D reconstruction accomplished in one step make it easy to combine different defocus data sets and decrease the error accumulation in the computation. These methods were applied to energy-filtered images of the 70S Escherichia coli ribosome, resulting in a distortion-free 3D map of the ribosome at 1/24.5 Angstrom(-1) resolution, as determined by the differential phase residual resolution criterion. (C) 1997 Academic Press.
引用
收藏
页码:197 / 219
页数:23
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