Chiral separation of homocysteine by derivatization with 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole followed by capillary electrophoresis using γ-cyclodextrin

被引：22

作者：

Kim, IJ ^{[1
]}

Park, SJ ^{[1
]}

Kim, HJ ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Mol Engn, Seoul 151742, South Korea

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 877卷 / 1-2期

关键词：

enantiomer separation; derivatization; electrophoresis; homocysteine; aminosulfonylfluorobenzoxadiazole; cyclodextrins; amino acids;

D O I：

10.1016/S0021-9673(00)00222-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Homocysteine was derivatized with 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F) to form an inclusion complex with cyclodextrin and to facilitate UV detection. ABD-homocysteine showed interaction with beta- and gamma-cyclodextrin in capillary electrophoresis at pH 2.25 as indicated by the decreased migration time. However, chiral separation of D,L-ABD-homocysteine was observed using gamma-CD only. Optimal separation was obtained at pH 2.25, 50 mM gamma-CD concentration, and 20 kV applied voltage. L-ABD-Homocysteine migrated faster than the D-isomer as demonstrated by a spiking experiment using dithiothreitol-reduced L-homocystine. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：217 / 223

页数：7

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