Amperometric detection of organic thiols at a tungsten wire electrode following their separation by liquid chromatography

被引：23

作者：

Hidayat, A

Hibbert, DB

Alexander, PW

机构：

[1] UNIV NEW S WALES,DEPT ANALYT CHEM,SYDNEY,NSW 2052,AUSTRALIA

[2] UNIV TASMANIA,DEPT PHYS SCI,LAUNCESTON,TAS 7001,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 693卷 / 01期

关键词：

tungsten wire electrode; thiols; cysteine; homocysteine; glutathione; reduced; penicillamine; 2-mercaptopropionic acid; thiourea;

D O I：

10.1016/S0378-4347(97)00054-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The amperometric detection of thiols following their separation by reversed-phase chromatography and reaction into a post-column mercury carrier stream is shown to be a sensitive method when using a tungsten wire sensor as the working electrode in a three-electrode flow cell. Five organic thiols (cysteine, homocysteine, reduced glutathione, D,L-penicillamine and 2-mercaptopropionic acid) and thiourea could be separated within approx. 18 min. The analytical performance is comparable, and stability superior, to chemically modified electrodes previously reported.

引用

页码：139 / 146

页数：8

共 23 条

[1] TUNGSTEN SENSOR FOR AMPEROMETRIC DETECTION OF ORGANIC THIOLS AND PROTEINS [J].