Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions

被引：23

作者：

Fedorova, O

Su, LJ

Pyle, AM

机构：

[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA

[2] Columbia Univ, Howard Hughes Med Inst, New York, NY 10032 USA

来源：

METHODS | 2002年 / 28卷 / 03期

关键词：

group II intron; kinetics; self-splicing; ribozyme;

D O I：

10.1016/S1046-2023(02)00239-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we present construct designs and protocols that were used to develop a set of kinetic frameworks for studying the structure and reaction mechanisms of group 11 introns and ribozymes derived from them. In addition, we discuss application of these systems to structure/function analysis of the ai5gamma group 11 intron. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：323 / 335

页数：13

共 78 条

[1] Catalytic role of 2'-hydroxyl groups within a group II intron active site [J].