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Group II introns: highly specific endonucleases with modular structures and diverse catalytic functions

被引：23
作者：
Fedorova, O
Su, LJ
Pyle, AM
机构：
[1] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY 10032 USA
[2] Columbia Univ, Howard Hughes Med Inst, New York, NY 10032 USA
来源：
METHODS | 2002年 / 28卷 / 03期
关键词：
group II intron; kinetics; self-splicing; ribozyme;
D O I：
10.1016/S1046-2023(02)00239-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Group II introns are large catalytic RNAs with a remarkable repertoire of reactions. Here we present construct designs and protocols that were used to develop a set of kinetic frameworks for studying the structure and reaction mechanisms of group 11 introns and ribozymes derived from them. In addition, we discuss application of these systems to structure/function analysis of the ai5gamma group 11 intron. (C) 2002 Elsevier Science (USA). All rights reserved.
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页码：323 / 335
页数：13
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