Bone marrow Derived Pluripotent Cells are Pericytes which Contribute to Vascularization

被引：67

作者：

Cai, Xiaoxiao ^{[1
,2
]}

Lin, Yunfeng ^{[1
,3
,4
,5
]}

Friedrich, Claudia C. ^{[3
]}

Neville, Craig ^{[3
]}

Pomerantseva, Irina ^{[3
]}

Sundback, Cathryn A. ^{[3
]}

Sharma, Parul ^{[2
]}

Zhang, Zhiyuan ^{[2
]}

Vacanti, Joseph P. ^{[3
]}

Hauschka, Peter V. ^{[2
]}

Grottkau, Brian E. ^{[4
,5
]}

机构：

[1] Sichuan Univ, W China Coll Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China

[2] Harvard Univ, Sch Med, Childrens Hosp Boston, Dept Orthopaed Surg, Boston, MA USA

[3] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Regenerat Med, Boston, MA 02114 USA

[4] Harvard Univ, Sch Med, Mass Gen Hosp Children, Dept Orthopaed Surg, Boston, MA USA

[5] Harvard Univ, Sch Med, Pediat Orthopaed Lab Tissue Engn, Boston, MA USA

来源：

STEM CELL REVIEWS AND REPORTS | 2009年 / 5卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Bone marrow stromal cells; Pericytes; Multilineage differentiation; Endothelial cells; Vascularization; PROGENITOR CELLS; STEM-CELLS; IN-VIVO; ENDOTHELIAL-CELLS; TISSUE; NETWORK; ALPHA; DIFFERENTIATION; IDENTIFICATION; VASCULATURE;

D O I：

10.1007/s12015-009-9097-6

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Pericytes are essential to vascularization, but the purification and characterization of pericytes remain unclear. Smooth muscle actin alpha (alpha-SMA) is one maker of pericytes. The aim of this study is to purify the alpha-SMA positive cells from bone marrow and study the characteristics of these cells and the interaction between alpha-SMA positive cells and endothelial cells. The bone marrow stromal cells were harvested from alpha-SMA-GFP transgenic mice, and the alpha-SMA-GFP positive cells were sorted by FACS. The proliferative characteristics and multilineage differentiation ability of the alpha-SMA-GFP positive cells were tested. A 3-D culture model was then applied to test their vascularization by loading alpha-SMA-GFP positive cells and endothelial cells on collagen-fibronectin gel. Results demonstrated that bone marrow stromal cells are mostly alpha-SMA-GFP positive cells which are pluripotent, and these cells expressed alpha-SMA during differentiation. The alpha-SMA-GFP positive cells could stimulate the endothelial cells to form tube-like structures and subsequently robust vascular networks in 3-D culture. In conclusion, the bone marrow derived pluripotent cells are pericytes and can contribute to vascularization.

引用

页码：437 / 445

页数：9

共 28 条

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