Sputnik: a database platform for comparative plant genomics

被引：35

作者：

Rudd, S

Mewes, HW

Mayer, KFX

机构：

[1] GSF, Natl Res Ctr Environm & Hlth, Inst Bioinformat MIPS, D-85764 Neuherberg, Germany

[2] Tech Univ Munich, Wissensch Zentrum Weihenstephan, Dept Genome Oriented Bioinformat, D-85350 Freising Weihenstephan, Germany

来源：

NUCLEIC ACIDS RESEARCH | 2003年 / 31卷 / 01期

关键词：

D O I：

10.1093/nar/gkg075

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two million plant ESTs, from 20 different plant species, and totalling more than one 1000 Mbp of DNA sequence, represents a formidable transcriptomic resource. Sputnik uses the potential of this sequence resource to fill some of the information gap in the un-sequenced plant genomes and to serve as the foundation for in silicio comparative plant genomics. The complexity of the individual EST collections has been reduced using optimised EST clustering techniques. Annotation of cluster sequences is performed by exploiting and transferring information from the comprehensive knowledge-base already produced for the completed model plant genome ( Arabidopsis thaliana) and by performing additional state of-the-art sequence analyses relevant to today's plant biologist. Functional predictions, comparative analyses and associative annotations for 500 000 plant EST derived peptides make Sputnik (http://mips.gsf.de/proj/sputnik/) a valid platform for contemporary plant genomics.

引用

页码：128 / 132

页数：5

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