Secondary structures and starvation-induced frameshifting

被引：8

作者：

Atkinson, J ^{[1
]}

Dodge, M ^{[1
]}

Gallant, J ^{[1
]}

机构：

[1] UNIV WASHINGTON, DEPT GENET, SEATTLE, WA 98195 USA

来源：

MOLECULAR MICROBIOLOGY | 1997年 / 26卷 / 04期

关键词：

D O I：

10.1046/j.1365-2958.1997.6101959.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the effect of a downstream secondary structure (the stem-loop sequence found downstream on the MMTV gag-pro frameshift site) on frameshifting at a bacterial shifty site (U UUC AUA) that responds strongly to a isoleucine-tRNA limitation. Our findings are as follows: (i) the downstream stem-loop has little effect on frameshifting in growing, unstarved cells; (ii) the stem-loop increases the frameshifting effect of isoleucine-tRNA limitation about fourfold, and this synergism is maximal with a distance of 5-9 nucleotides between the 'hungry' AUA codon and the stem-loop; and (iii) a stem-loop of different sequence at the same position has the same effect.

引用

收藏

页码：747 / 753

页数：7

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