Comparative evaluation of two different methods of inoculum preparation for antifungal susceptibility testing of filamentous fungi

被引：73

作者：

Aberkane, A ^{[1
]}

Cuenca-Estrella, M ^{[1
]}

Gomez-Lopez, A ^{[1
]}

Petrikkou, E ^{[1
]}

Mellado, E ^{[1
]}

Monzón, A ^{[1
]}

Rodriguez-Tudela, JL ^{[1
]}

机构：

[1] Inst Salud Carlos III, Serv Micol, Ctr Nacl Microbiol, Madrid 28220, Spain

来源：

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | 2002年 / 50卷 / 05期

关键词：

D O I：

10.1093/jac/dkf187

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Two different methods of inoculum preparation for susceptibility testing were analysed. The first method was adjustment of inoculum size by haemocytometer counting. The second method was spectrophotometric adjustment at 530 nm. The reliability of both methods was assessed by colony counting. The overall agreement between colony counting and haemocytometer counts was 93.6%, and the intraclass coefficient correlation was 0.71 (P < 0.05). Pearson's correlation index between colony counts and optical density values was -0.059 (P > 0.05). Optical densities ranged between 0.01 and 1.2, showing less reproducibility than expected by the NCCLS M38-P standard. Haemocytometer counting is a more reliable method of inoculum preparation for antifungal susceptibility testing.

引用

页码：719 / 722

页数：4

共 10 条

[1] Comparative in-vitro activity of voriconazole (UK-109,496) and six other antifungal agents against clinical isolates of Scedosporium prolificans and Scedosporium apiospermum
Cuenca-Estrella, M
Ruiz-Díez, B
Martínez-Suárez, JV
Monzón, A
Rodríguez-Tudela, JL
[J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1999, 43 (01) : 149 - 151
[2] de Hoog G.S., 2000, ATLAS CLIN FUNGI, VSecond
[3] Invasive aspergillosis
Denning, DW
[J]. CLINICAL INFECTIOUS DISEASES, 1998, 26 (04) : 781 - 803
[4] In vitro fungicidal activities of voriconazole, itraconazole, and amphotericin B against opportunistic moniliaceous and dematiaceous fungi
Espinel-Ingroff, A
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (03) : 954 - 958
[5] Germinated and nongerminated conidial suspensions for testing of susceptibilities of Aspergillus spp. to amphotericin B, itraconazole, posaconazole, ravuconazole, and voriconazole
Espinel-Ingroff, A
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2001, 45 (02) : 605 - 607
[6] SPECTROPHOTOMETRIC METHOD OF INOCULUM PREPARATION FOR THE INVITRO SUSCEPTIBILITY TESTING OF FILAMENTOUS FUNGI
ESPINELINGROFF, A
KERKERING, TM
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (02) : 393 - 394
[7] Antifungal drug resistance in Aspergillus
Moore, CB
Sayers, N
Mosquera, J
Slaven, J
Denning, DW
[J]. JOURNAL OF INFECTION, 2000, 41 (03) : 203 - 220
[8] National Committee for Clinical Laboratory Standards, 1998, M38P NAT COMM CLIN L
[9] Inoculum standardization for antifungal susceptibility testing of filamentous fungi pathogenic for humans
Petrikkou, E
Rodríguez-Tudela, JL
Cuenca-Estrella, M
Gómez, A
Molleja, A
Mellado, E
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (04) : 1345 - 1347
[10] Antifungal susceptibility testing: Practical aspects and current challenges
Rex, JH
Pfaller, MA
Walsh, TJ
Chaturvedi, V
Espinel-Ingroff, A
Ghannoum, MA
Gosey, LL
Odds, FC
Rinaldi, MG
Sheehan, DJ
Warnock, DW
[J]. CLINICAL MICROBIOLOGY REVIEWS, 2001, 14 (04) : 643 - 658

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