The VP16 activation domain establishes an active mediator lacking CDK8 in vivo

被引:36
作者
Uhlmann, Thomas [1 ]
Boeing, Stefan [1 ]
Lehmbacher, Michael [1 ]
Meisterernst, Michael [1 ]
机构
[1] Natl Res Ctr Environm & Hlth, D-81377 Munich, Germany
关键词
D O I
10.1074/jbc.M608451200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
VP16 has been widely used to unravel the mechanisms underlying gene transcription. Much of the previous work has been conducted in reconstituted in vitro systems. Here we study the formation of transcription complexes at stable reporters under the control of an inducible Tet-VP16 activator in living cells. In this simplified model for gene activation VP16 recruits the general factors and the cofactors Mediator, GCN5, CBP, and PC4, within minutes to the promoter region. Activation is accompanied by only minor changes in histone acetylation and H3K4 methylation but induces a marked promoter-specific increase in H3K79 methylation. Mediated through contacts with VP16 several subunits of the cleavage and polyadenylation factor (CPSF/CstF) are concentrated at the promoter region. We provide in vitro and in vivo evidence that VP16 activates transcription through a specific MED25-associated Mediator, which is deficient in CDK8.
引用
收藏
页码:2163 / 2173
页数:11
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