A mediator-free amperometric hydrogen peroxide biosensor based on HRP immobilized on a nano-Au/poly 2,6-pyridinediamine-coated electrode

被引：38

作者：

Cao, Shurui ^{[1
]}

Yuan, Ruo ^{[1
]}

Chai, Yaqin ^{[1
]}

Zhang, Lingyan ^{[1
]}

Li, Xuelian ^{[1
]}

Gao, Fengxian ^{[1
]}

机构：

[1] Southwest Univ, Coll Chem & Chem Engn, Chongqing Key Lab Analyt Chem, Chongqing 400715, Peoples R China

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2007年 / 30卷 / 02期

基金：

中国国家自然科学基金;

关键词：

2,6-pyridinediamine; colloidal Au; horseradish peroxidase; biosensor; electropolymerization;

D O I：

10.1007/s00449-006-0100-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A mediator-free amperometric hydrogen peroxide biosensor was prepared by immobilizing horseradish peroxidase (HRP) enzyme on colloidal Au modified platinum (Pt) wire electrode, which was modified by poly 2,6-pyridinediamine (pPA). The modified process was characterized by electrochemical impedance spectroscopy (EIS), and the electrochemical characteristics of the biosensor were studied by cyclic voltammetry, linear sweep voltammetry and chronoamperometry. The biosensor displayed an excellent electrocatalytical response to reduction of H2O2 without the aid of an electron mediator, the linear range was 4.2 x 10(-7)-1.5 x 10(-3) mol/L (r = 0.9977), with a detection limit of 1.4 x 10(-7) mol/L. Moreover, the performance and factors influencing the resulted biosensor were studied in detail. The studied biosensor exhibited permselectivity, good stability and good fabrication reproducibility.

引用

页码：71 / 78

页数：8

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