Kinetics of H+ ion binding by the P(+)Q(A-) state of bacterial photosynthetic reaction centers: Rate limitation within the protein

被引:60
作者
Maroti, P
Wraight, CA
机构
[1] UNIV ILLINOIS, DEPT PLANT BIOL, URBANA, IL 61801 USA
[2] UNIV ILLINOIS, CTR BIOPHYS & COMPUTAT BIOL, URBANA, IL 61801 USA
[3] ATTILA JOZSEF UNIV, INST BIOPHYS, H-6722 SZEGED, HUNGARY
基金
美国国家科学基金会;
关键词
D O I
10.1016/S0006-3495(97)78077-9
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The kinetics of flash-induced H(+) ion binding by isolated reaction centers (RCs) of Rhodobacter sphaeroides, strain R-26, were measured, using pH indicators and conductimetry, in the presence of terbutryn to block electron transfer between the primary and secondary quinones (Q(A) and Q(B)), and in the absence of exogenous electron donors to the oxidized primary donor, P(+), i.e., the P(+)Q(A)(-) state. Under these conditions, proton binding by RCs is to the protein rather than to any of the cofactors. After light activation to form P(+)Q(A)(-), the kinetics of proton binding were monoexponential at all pH values studied. At neutral pH, the apparent bimolecular rate constant was close to the diffusional limit for proton transfer in aqueous solution (similar to 10(11)M(-1)s(-1)), but increased significantly in the alkaline pH range (e.g., 2 x 10(13)M(-1)s(-1) at pH 10). The average slope of the pH dependence was -0.4 instead of -1.0, as might be expected for a H(+) diffusion-controlled process. High activation energy (0.54 eV at pH 8.0) and weak viscosity dependence showed that H(+) ion uptake by RCs is not limited by diffusion. The salt dependence of the H(+) ion binding rate and the pK values of the protonatable amino acid residues of the reaction center implicated surface charge influences, and Gouy-Chapman theory provided a workable description of the ionic effects as arising from modulation of the pH at the surface of the RC. Incubation in D(2)O caused small increases in the pKs of the protonatable groups and a small, pH (pD)-dependent slowing of the binding rate, The salt, pH, temperature, viscosity, and D(2)O dependences of the proton uptake by RCs in the P(+)Q(A)(-) state were accounted for by three considerations: 1) parallel pathways of H(+) delivery to the RC, contributing to the observed (net) H(+) disappearance; 2) rate limitation of the protonation of target groups within the protein by conformational dynamics; and 3) electrostatic influences of charged groups in the protein, via the surface pH.
引用
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页码:367 / 381
页数:15
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