Immature dendritic cells (CD1 1c+CD3-B220- cells) present in mouse peripheral blood

It is well known that dendritic cells (DCs) are developed from the peripheral blood of mice when peripheral blood mononuclear cells (PBMCs) are cultured with GM-CSF. We have previously found that immature DCs are present in the blood even in humans. In the present study, we show that CD11c(+)CD3(-)B220(-) cells in the mouse peripheral blood are immature DCs. The percentage of CD11c(+)CD3(-)B220(-) cells in the (PBMCs) of normal mice ranges from 0.5 to 2.5%. The CD11c(+)CD3(-)B220(-) cells in the PBMCs show dendrites, similar in shape to the CD11c(+)CD3(-)B220(-) cells in the spleen, which are thought to be DCs definitely. However, they have practically no capacity to stimulate the proliferation of allogeneic T cells, and show a lower expression of MHC class II, B7-1 and B7-2 than CD11c(+)CD3(-)B220(-) cells in the spleen. When the CD11c(+)CD3(-)B220(-) cells in the PBMCs are cultured with GM-CSF, they show not only the potent ability to stimulate the proliferation of allogeneic T cells but also a higher expression of MHC class II, B7-1 and B7-2. Moreover, they migrate into the spleen when they are injected intravenously. These results suggest that CD11c(+)CD3(-)B220(-) cells in the PBMCs are immature DCs, and that they migrate into the spleen, where they mature.