Intermediates: ubiquitous species on folding energy landscapes?

被引:175
作者
Brockwell, David J. [1 ]
Radford, Sheena E. [1 ]
机构
[1] Univ Leeds, Inst Mol & Cellular Biol, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England
基金
英国生物技术与生命科学研究理事会; 英国惠康基金;
关键词
D O I
10.1016/j.sbi.2007.01.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although intermediates have long been recognised as fascinating species that form during the folding of large proteins, the role that intermediates play in the folding of small, single-domain proteins has been widely debated. Recent discoveries using new, sensitive methods of detection and studies combining simulation and experiment have now converged on a common vision for folding, involving intermediates as ubiquitous stepping stones en route to the native state. The results suggest that the folding energy landscapes of even the smallest proteins possess significant ruggedness in which intermediates stabilized by both native and non-native interactions are common features.
引用
收藏
页码:30 / 37
页数:8
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