Localisation and regulation of the eIF4E-binding protein 4E-BP3

被引：21

作者：

Kleijn, M ^{[1
]}

Scheper, GC ^{[1
]}

Wilson, ML ^{[1
]}

Tee, AR ^{[1
]}

Proud, CG ^{[1
]}

机构：

[1] Univ Dundee, Sch Life Sci, Div Mol Physiol, Dundee DD1 5EH, Scotland

来源：

FEBS LETTERS | 2002年 / 532卷 / 03期

基金：

英国医学研究理事会;

关键词：

eIF4E-binding protein 3; eIF4E-binding protein 1; primary T cell; regulation; nucleus;

D O I：

10.1016/S0014-5793(02)03694-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cap-binding protein eIF4E-binding protein 3 (4E-BP3) was identified some years ago, but its properties have not been investigated in detail. In this report, we investigated the regulation and localisation of 4E-BP3. We show that 4E-BP3 is present in the nucleus as well as in the cytoplasm in primary T cells, HEK293 cells and HeLa cells. 4E-BP3 was associated with eIF4E in both cell compartments. Furthermore, 4E-BP3/eIF4E association in the cytoplasm was regulated by serum or interleukin-2 starvation in the different cell types. Rapamycin did not affect the association of eIF4E with 4E-BP3 in the cytoplasm or in the nucleus. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：319 / 323

页数：5

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