Effect of soil bradyrhizobia on the success of soybean inoculant strain CB 1809

被引:26
作者
Botha, WJ
Jaftha, JB
Bloem, JF
Habig, JH
Law, IJ
机构
[1] ARC, Plant Protect Res Inst, ZA-0121 Queenswood, South Africa
[2] Univ Pretoria, Dept Microbiol & Plant Pathol, ZA-0001 Pretoria, South Africa
关键词
Bradyrhizobium; serotypes; inoculation; soybean;
D O I
10.1016/j.micres.2004.04.004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Four decades of soybean [Glycine max (L.) Merr.] cultivation in South Africa has resulted in the establishment of populations of bradyrhizobia against which the recently introduced inoculant strain CB 1809 must compete. Serological and DNA fingerprinting methods were used to studs the diversity of nodule isolates from soils at Bergville, Koedoeskop and Morgenzon. Dominant serogroups included Bradyrhizobium elkanii serotype 76 at Bergville (67%), Bradyrhizobium japonicum serotype 123 at Morgenzon (81%) and B. japonicum serotype 135 at Koedoeskop (100%). Their origin is unknown as they do not correspond in serotype to strains used in previous inoculants. A small percentage of isolates from Bergville (13%) and Morgenzon (16%) were serologically homologous to strain WB 1 (serotype 31/76), applied for two decades before CB 1809 (serotype 122). Nitrogen-fixing effectiveness of CB 1809 was superior to 60% of the isolates tested from Bergville and Morgenzon, but similar to 73% of the Koedoeskop isolates. Seed and liquid-in-furrow application methods increased CB 1809 nodule occupancy at least three-fold above background Levels at Bergville (pH 5.16) and Morgenzon (pH 6.33). Inoculation did not, however, increase CB 1809 nodule occupancy at Koedoeskop (pH 7.76), possibly because alkaline soil conditions favoured the serotype 135 population predominant at this site. (C) 2004 Elsevier GmbH. All rights reserved.
引用
收藏
页码:219 / 231
页数:13
相关论文
共 49 条
[41]   INFLUENCE OF THE SIZE OF INDIGENOUS RHIZOBIAL POPULATIONS ON ESTABLISHMENT AND SYMBIOTIC PERFORMANCE OF INTRODUCED RHIZOBIA ON FIELD-GROWN LEGUMES [J].
THIES, JE ;
SINGLETON, PW ;
BENBOHLOOL, B .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (01) :19-28
[42]   ENVIRONMENTAL-EFFECTS ON COMPETITION FOR NODULE OCCUPANCY BETWEEN INTRODUCED AND INDIGENOUS RHIZOBIA AND AMONG INTRODUCED STRAINS [J].
THIES, JE ;
BENBOHLOOL, B ;
SINGLETON, PW .
CANADIAN JOURNAL OF MICROBIOLOGY, 1992, 38 (06) :493-500
[43]   Evolutionary relationships among the soybean bradyrhizobia reconstructed from 16S rRNA gene and internally transcribed spacer region sequence divergence [J].
van Berkum, P ;
Fuhrmann, JJ .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :2165-2172
[44]  
Van Rensburg HJ, 1976, PHYTOPHYLACTICA, V8, P91
[45]   EFFECTIVENESS OF RHIZOBIUM STRAINS USED IN INOCULANTS AFTER THEIR INTRODUCTION INTO SOIL [J].
VANRENSBURG, HJ ;
STRIJDOM, BW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1985, 49 (01) :127-131
[46]  
VINCENT J. M., 1970, A manual for the practical study of the root-nodule bacteria.
[47]  
Vinuesa P, 1998, APPL ENVIRON MICROB, V64, P2096
[48]   SEROLOGICAL DISTRIBUTION OF BRADYRHIZOBIUM-JAPONICUM FROM UNITED-STATES SOYBEAN PRODUCTION AREAS [J].
WEBER, DF ;
KEYSER, HH ;
URATSU, SL .
AGRONOMY JOURNAL, 1989, 81 (05) :786-789
[49]   Comparison of sequence analysis of 16S-23S rDNA spacer regions, AFLP analysis and DNA-DNA hybridizations in Bradyrhizobium [J].
Willems, A ;
Coopman, R ;
Gillis, M .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :623-632