Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells

被引：373

作者：

Sun, Ning ^{[2
]}

Panetta, Nicholas J. ^{[1
]}

Gupta, Deepak M. ^{[1
]}

Wilson, Kitchener D. ^{[2
]}

Lee, Andrew ^{[2
]}

Jia, Fangjun ^{[2
]}

Hu, Shijun ^{[2
]}

Cherry, Athena M. ^{[3
]}

Robbins, Robert C. ^{[4
,5
]}

Longaker, Michael T. ^{[1
,6
]}

Wu, Joseph C. ^{[2
,5
,7
]}

机构：

[1] Stanford Univ, Sch Med, Dept Surg, Div Plast & Reconstruct Surg, Stanford, CA 94305 USA

[2] Stanford Univ, Sch Med, Dept Radiol, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Med, Dept Pathol, Stanford, CA 94305 USA

[4] Stanford Univ, Sch Med, Dept Cardiothorac Surg, Stanford, CA 94305 USA

[5] Stanford Univ, Sch Med, Cardiovasc Inst Med, Stanford, CA 94305 USA

[6] Stanford Univ, Sch Med, Inst Stem Cell Biol & Regenerat Med, Stanford, CA 94305 USA

[7] Stanford Univ, Sch Med, Dept Med, Stanford, CA 94305 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 37期

基金：

美国国家卫生研究院;

关键词：

differentiation; pluripotency; reprogramming; KRUPPEL-LIKE FACTOR-5; HUMAN SOMATIC-CELLS; HUMAN FIBROBLASTS; GENERATION; INDUCTION; MOUSE;

D O I：

10.1073/pnas.0908450106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here that induced pluripotent stem (iPS) cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients. Furthermore, iPS cells can be readily derived from adult hASCs in a feeder-free condition, thereby eliminating potential variability caused by using feeder cells. hASCs can be safely and readily isolated from adult humans in large quantities without extended time for expansion, are easy to maintain in culture, and therefore represent an ideal autologous source of cells for generating individual-specific iPS cells.

引用

页码：15720 / 15725

页数：6

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