Cloning of a single-copy DNA sequence unique to Plasmodiophora brassicae

被引：39

作者：

Ito, S ^{[1
]}

Maehara, T ^{[1
]}

Tanaka, S ^{[1
]}

KameyaIwaki, M ^{[1
]}

Yano, S ^{[1
]}

Kishi, F ^{[1
]}

机构：

[1] YAMAGUCHI UNIV,CTR GENE RES,UBE,YAMAGUCHI 755,JAPAN

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 1997年 / 50卷 / 05期

关键词：

D O I：

10.1006/pmpp.1997.0087

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A 1.4 kb DNA fragment was amplified from Plasmodiophora brassicae by the polymerase chain reaction using a primer PBTZS-2 (5'-CCGAATTCGCGTCAGCGTGA-3'). The 1.4 kb fragment was found in all isolates examined in this study. Southern blot analysis showed that the DNA sequence existed as a single copy per haploid genome of P. brassicae. The 1.4 kb fragment was cloned and sequenced. Database searches did not reveal any significant homologies with previously characterized nucleotide sequences. A nested primer pair PBTZS-3 (5'-CCACGTCGATCACGTTGCAAT-3') and PBTZS-4 (5'-GCTGGCGTTGATGTACTGGAATT-3') derived from the fragment produced a single 398 bp product from ail isolates of P. brassicae and no products from other fungi and bacteria examined. The potential use of the primers (PBTZS-2, PBTZS-3, and PBTZS-4) for specific detection of P. brassicae is discussed. A method for preparation of high molecular weight DNA from lyophilized resting spores of P. brassicae is also described. (C) 1997 Academic Press Limited.

引用

页码：289 / 300

页数：12

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