Rapid and specific detection of Asian- and African-lineage Zika viruses

被引：79

作者：

Chotiwan, Nunya ^{[1
,2
]}

Brewster, Connie D. ^{[1
]}

Magalhaes, Tereza ^{[2
,3
]}

Weger-Lucarelli, James ^{[1
,2
]}

Duggal, Nisha K. ^{[4
]}

Ruckert, Claudia ^{[1
,2
]}

Chilinh Nguyen ^{[1
,2
]}

Luna, Selene M. Garcia ^{[1
,2
]}

Fauver, Joseph R. ^{[1
,2
]}

Andre, Barb ^{[1
]}

Gray, Meg ^{[1
,2
]}

Black, William C. ^{[1
,2
]}

Kading, Rebekah C. ^{[1
,2
]}

Ebel, Gregory D. ^{[1
,2
]}

Kuan, Guillermina ^{[5
]}

Balmaseda, Angel ^{[6
]}

Jaenisch, Thomas ^{[7
,8
]}

Marques, Ernesto T. A. ^{[3
,9
]}

Brault, Aaron C. ^{[4
]}

Harris, Eva ^{[10
]}

Foy, Brian D. ^{[1
,2
]}

Quackenbush, Sandra L. ^{[1
]}

Perera, Rushika ^{[1
,2
]}

Rovnak, Joel ^{[1
]}

机构：

[1] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

[2] Colorado State Univ, Coll Vet Med & Biomed Sci, Arthropod Borne Infect Dis Labs, Ft Collins, CO 80523 USA

[3] Fundacao Oswaldo Cruz, Ctr Pesquisas Aggeu Magalhaes, Lab Virol & Expt Therapeut, Recife, PE, Brazil

[4] Ctr Dis Control & Prevent, Div Vector Borne Dis, Ft Collins, CO 80521 USA

[5] Minist Hlth, Ctr Salud Socrates Flores Vivas, Managua, Nicaragua

[6] Minist Hlth, Ctr Nacl Diagnost & Referencia, Lab Nacl Virol, Managua, Nicaragua

[7] Heidelberg Univ Hosp, Dept Infect Dis, Sect Clin Trop Med, Heidelberg, Germany

[8] German Ctr Infect Res DZIF, Partner Site Heidelberg, Heidelberg, Germany

[9] Univ Pittsburgh, Sch Publ Hlth, Ctr Vaccine Res, Pittsburgh, PA 15261 USA

[10] Univ Calif Berkeley, Sch Publ Hlth, Div Infect Dis & Vaccinol, Berkeley, CA 94720 USA

来源：

SCIENCE TRANSLATIONAL MEDICINE | 2017年 / 9卷 / 388期

关键词：

MEDIATED ISOTHERMAL AMPLIFICATION; REAL-TIME DETECTION; CHIKUNGUNYA-VIRUS; DENGUE VIRUS; CLINICAL-EVALUATION; FRENCH-POLYNESIA; TRANSMISSION; ASSAY; IDENTIFICATION; PHYLOGENY;

D O I：

10.1126/scitranslmed.aag0538

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.

引用

页数：13

共 50 条

[1] Application of molecular typing techniques in the 1998 dengue epidemic in Nicaragua
Balmaseda, A
Sandoval, E
Pérez, L
Gutiérrez, CM
Harris, E
[J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1999, 61 (06) : 893 - 897
[2] BEARCROFT W. G. C., 1956, TRANS ROY SOC TROP MED AND HYG, V50, P442, DOI 10.1016/0035-9203(56)90090-6
[3] Evidence of perinatal transmission of Zika virus, French Polynesia, December 2013 and February 2014
Besnard, M.
Lastere, S.
Teissier, A.
Cao-Lormeau, V. M.
Musso, D.
[J]. EUROSURVEILLANCE, 2014, 19 (13): : 13 - 16
[4] Zika Virus as a Cause of Neurologic Disorders
Broutet, Nathalie
Krauer, Fabienne
Riesen, Maurane
Khalakdina, Asheena
Almiron, Maria
Aldighieri, Sylvain
Espinal, Marcos
Low, Nicola
Dye, Christopher
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 2016, 374 (16) : 1506 - 1509
[5] Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study
Calvet, Guilherme
Aguiar, Renato S.
Melo, Adriana S. O.
Sampaio, Simone A.
de Filippis, Ivano
Fabri, Allison
Araujo, Eliane S. M.
de Sequeira, Patricia C.
de Mendonca, Marcos C. L.
de Oliveira, Louisi
Tschoeke, Diogo A.
Schrago, Carlos G.
Thompson, Fabiano L.
Brasil, Patricia
dos Santos, Flavia B.
Nogueira, Rita M. R.
Tanurit, Amilcar
de Filippis, Ana M. B.
[J]. LANCET INFECTIOUS DISEASES, 2016, 16 (06) : 653 - 660
[6] Results of a Zika Virus (ZIKV) Immunoglobulin M-Specific Diagnostic Assay Are Highly Correlated With Detection of Neutralizing Anti-ZIKV Antibodies in Neonates With Congenital Disease
Cordeiro, Marli T.
Brito, Carlos A. A.
Pena, Lindomar J.
Castanha, Priscila M. S.
Gil, Laura H. V. G.
Lopes, Kennya G. S.
Dhalia, Rafael
Meneses, Jucille A.
Ishigami, Ana C.
Mello, Luisa M.
Alencar, Liciana X. E.
Guarines, Klarissa M.
Rodrigues, Laura C.
Marques, Ernesto T. A.
[J]. JOURNAL OF INFECTIOUS DISEASES, 2016, 214 (12) : 1897 - 1904
[7] ZIKA VIRUS .1. ISOLATIONS AND SEROLOGICAL SPECIFICITY
DICK, GWA
KITCHEN, SF
HADDOW, AJ
[J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1952, 46 (05) : 509 - 520
[8] Zika virus in the Americas: Early epidemiological and genetic findings
Faria, Nuno Rodrigues
da Silva Azevedo, Raimunda do Socorro
Kraemer, Moritz U. G.
Souza, Renato
Cunha, Mariana Sequetin
Hill, Sarah C.
Theze, Julien
Bonsall, Michael B.
Bowden, Thomas A.
Rissanen, Ilona
Rocco, Iray Maria
Nogueira, Juliana Silva
Maeda, Adriana Yurika
da Silva Vasami, Fernanda Giseli
de Lima Macedo, Fernando Luiz
Suzuki, Akemi
Rodrigues, Sueli Guerreiro
Ribeiro Cruz, Ana Cecilia
Nunes, Bruno Tardeli
de Almeida Medeiros, Daniele Barbosa
Guerreiro Rodrigues, Daniela Sueli
Nunes Queiroz, Alice Louize
Pinto da Silva, Eliana Vieira
Henriques, Daniele Freitas
Travassos da Rosa, Elisabeth Salbe
de Oliveira, Consuelo Silva
Martins, Livia Caricio
Vasconcelos, Helena Baldez
Neves Casseb, Livia Medeiros
Simith, Darlene de Brito
Messina, Jane P.
Abade, Leandro
Loureno, Jose
Junior Alcantara, Luiz Carlos
de Lima, Maricelia Maia
Giovanetti, Marta
Hay, Simon I.
de Oliveira, Rodrigo Santos
Lemos, Poliana da Silva
de Oliveira, Layanna Freitas
Silva de Lima, Clayton Pereira
da Silva, Sandro Patroca
de Vasconcelos, Janaina Mota
Franco, Luciano
Cardoso, Jedson Ferreira
da Silva Gonalves Vianez-Junior, Joao Lidio
Mir, Daiana
Bello, Gonzalo
Delatorre, Edson
Khan, Kamran
[J]. SCIENCE, 2016, 352 (6283) : 345 - 349
[9] Probable Non-Vector-borne Transmission of Zika Virus, Colorado, USA
Foy, Brian D.
Kobylinski, Kevin C.
Foy, Joy L. Chilson
Blitvich, Bradley J.
da Rosa, Amelia Travassos
Haddow, Andrew D.
Lanciotti, Robert S.
Tesh, Robert B.
[J]. EMERGING INFECTIOUS DISEASES, 2011, 17 (05) : 880 - 882
[10] Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue
Goto, Motoki
Honda, Eiichi
Ogura, Atsuo
Nomoto, Akio
Hanaki, Ken-Ichi
[J]. BIOTECHNIQUES, 2009, 46 (03) : 167 - +

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