Rapid and specific detection of Asian- and African-lineage Zika viruses

被引：79

作者：

Chotiwan, Nunya ^{[1
,2
]}

Brewster, Connie D. ^{[1
]}

Magalhaes, Tereza ^{[2
,3
]}

Weger-Lucarelli, James ^{[1
,2
]}

Duggal, Nisha K. ^{[4
]}

Ruckert, Claudia ^{[1
,2
]}

Chilinh Nguyen ^{[1
,2
]}

Luna, Selene M. Garcia ^{[1
,2
]}

Fauver, Joseph R. ^{[1
,2
]}

Andre, Barb ^{[1
]}

Gray, Meg ^{[1
,2
]}

Black, William C. ^{[1
,2
]}

Kading, Rebekah C. ^{[1
,2
]}

Ebel, Gregory D. ^{[1
,2
]}

Kuan, Guillermina ^{[5
]}

Balmaseda, Angel ^{[6
]}

Jaenisch, Thomas ^{[7
,8
]}

Marques, Ernesto T. A. ^{[3
,9
]}

Brault, Aaron C. ^{[4
]}

Harris, Eva ^{[10
]}

Foy, Brian D. ^{[1
,2
]}

Quackenbush, Sandra L. ^{[1
]}

Perera, Rushika ^{[1
,2
]}

Rovnak, Joel ^{[1
]}

机构：

[1] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

[2] Colorado State Univ, Coll Vet Med & Biomed Sci, Arthropod Borne Infect Dis Labs, Ft Collins, CO 80523 USA

[3] Fundacao Oswaldo Cruz, Ctr Pesquisas Aggeu Magalhaes, Lab Virol & Expt Therapeut, Recife, PE, Brazil

[4] Ctr Dis Control & Prevent, Div Vector Borne Dis, Ft Collins, CO 80521 USA

[5] Minist Hlth, Ctr Salud Socrates Flores Vivas, Managua, Nicaragua

[6] Minist Hlth, Ctr Nacl Diagnost & Referencia, Lab Nacl Virol, Managua, Nicaragua

[7] Heidelberg Univ Hosp, Dept Infect Dis, Sect Clin Trop Med, Heidelberg, Germany

[8] German Ctr Infect Res DZIF, Partner Site Heidelberg, Heidelberg, Germany

[9] Univ Pittsburgh, Sch Publ Hlth, Ctr Vaccine Res, Pittsburgh, PA 15261 USA

[10] Univ Calif Berkeley, Sch Publ Hlth, Div Infect Dis & Vaccinol, Berkeley, CA 94720 USA

来源：

SCIENCE TRANSLATIONAL MEDICINE | 2017年 / 9卷 / 388期

关键词：

MEDIATED ISOTHERMAL AMPLIFICATION; REAL-TIME DETECTION; CHIKUNGUNYA-VIRUS; DENGUE VIRUS; CLINICAL-EVALUATION; FRENCH-POLYNESIA; TRANSMISSION; ASSAY; IDENTIFICATION; PHYLOGENY;

D O I：

10.1126/scitranslmed.aag0538

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.

引用

页数：13

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