Molecular imaging of amyloid β peptides in mouse brain sections using mass spectrometry

被引：180

作者：

Stoeckli, M ^{[1
]}

Staab, D ^{[1
]}

Staufenbiel, M ^{[1
]}

Wiederhold, KH ^{[1
]}

Signor, L ^{[1
]}

机构：

[1] Novartis Pharma AG, RES CT AIS MS, CH-4002 Basel, Switzerland

来源：

ANALYTICAL BIOCHEMISTRY | 2002年 / 311卷 / 01期

关键词：

D O I：

10.1016/S0003-2697(02)00386-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is presented for direct spatial analysis of amyloid beta peptides in biological tissue sections. The technique takes advantage of the very high sensitivity of matrix-assisted laser desorption/ionization mass spectrometry and is implemented on a commercial instrument with modifications to only a few components and the software. With this setup, hundreds of molecular images can be generated simultaneously and within just a few minutes. The current features are an instrumental resolution of 50 mum and a sensitivity down to the attomol range. This new technology is applied to the study of amyloid P peptide distribution in brain sections from mice, showing features reminiscent of Alzheimer's disease. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：33 / 39

页数：7

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