Hairpin DNA probe based electrochemical biosensor using methylene blue as hybridization indicator

被引：137

作者：

Jin, Yan ^{[1
]}

Yao, Xin ^{[1
]}

Liu, Qing ^{[1
]}

Li, Jinghong ^{[1
]}

机构：

[1] Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2007年 / 22卷 / 06期

基金：

中国国家自然科学基金;

关键词：

hairpin DNA; electrochemical biosensor; methylene blue;

D O I：

10.1016/j.bios.2006.04.011

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this paper, a label-free, rapid and simple method was proposed to study the hybridization specificity of hairpin DNA probe using methylene blue (MB) as a hybridization indicator. Thiolated hairpin DNA probe was immobilized on the gold electrode by self-assembly. The voltammetric signals of MB were investigated at these modified electrodes by means of cyclic voltammetry (CV) detection. Single-base mutation oligonucleotide and random oligonucleotide can be easily discriminated from complementary target DNA. The effect of mismatch position in target DNA was investigated. Experimental results showed that mutation in the center of target DNA had greatest effect on the hybridization with hairpin DNA probe. The relationship between electrochemical responses and DNA target concentration was also studied. The reduction current of MB intercalation decreased with increasing the concentration of target DNA. Taken together, these experiments demonstrate that the hybridization indicator MB provides great promise for rapid and specific measurement of target DNA. 2006 Elsevier B.V. All rights reserved.

引用

页码：1126 / 1130

页数：5

共 26 条

[1] Mutation detection by electrocatalysis at DNA-modified electrodes [J].