In-Stem Molecular Beacon Containing a Pseudo Base Pair of Threoninol Nucleotides for the Removal of Background Emission

被引：55

作者：

Kashida, Hiromu ^{[1
]}

Takatsu, Tomohiko ^{[1
]}

Fujii, Taiga ^{[1
]}

Sekiguchi, Koji ^{[1
]}

Liang, Xingguo ^{[1
]}

Niwa, Kosuke ^{[3
]}

Takase, Tomokazu ^{[3
]}

Yoshida, Yasuko ^{[3
,4
]}

Asanuma, Hiroyuki ^{[1
,2
]}

机构：

[1] Nagoya Univ, Grad Sch Engn, Chikusa Ku, Nagoya, Aichi 4648603, Japan

[2] CREST Japan Sci & Technol Agcy, Kawaguchi, Saitama 3320012, Japan

[3] NGK Insulators Ltd, Mizuho Ku, Nagoya, Aichi 4678530, Japan

[4] Nagoya Univ, MEXT Innovat Res Ctr PME, Chikusa Ku, Nagoya, Aichi 4648603, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 38期

基金：

日本科学技术振兴机构;

关键词：

anthraquinones; DNA; fluorescent probes; molecular beacons; perylenes; NUCLEIC-ACID PROBES; REAL-TIME DETECTION; MISMATCH DETECTION; DNA STEM; HYBRIDIZATION; RNA; POLYMORPHISMS; SELECTIVITY; BIOSENSORS; CONJUGATE;

D O I：

10.1002/anie.200902367

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Off means off: An in-stem molecular beacon in which Dthreoninol units tether perylene and anthraquinone in the stem region effectively detected target sequences and was able to discriminate a one-base-deletion mutant from the wild-type (full-match) sequence without background emission (see picture). © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：7044 / 7047

页数：4

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