Detection of Cryptococcus by conventional, serological and molecular methods

被引:45
作者
Saha, Dolan Champa [1 ]
Xess, Immaculata [1 ]
Biswas, Ashutosh [2 ]
Bhowmik, Dipankar M. [3 ]
Padma, M. V. [4 ]
机构
[1] All India Inst Med Sci, Dept Microbiol, New Delhi 110029, India
[2] All India Inst Med Sci, Dept Med, New Delhi 110029, India
[3] All India Inst Med Sci, Dept Nephrol, New Delhi 110029, India
[4] All India Inst Med Sci, Dept Neurol, New Delhi 110029, India
关键词
LATEX AGGLUTINATION-TEST; HUMAN-IMMUNODEFICIENCY-VIRUS; CEREBROSPINAL-FLUID; ENZYME-IMMUNOASSAY; NEOFORMANS ANTIGEN; POLYSACCHARIDE ANTIGEN; PCR; TESTS; IDENTIFICATION; MENINGITIS;
D O I
10.1099/jmm.0.007328-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The rising incidence of cryptococcosis in India is posing a serious threat. Due to lack of sensitive methods for diagnosis, high morbidity and mortality are associated with the disease. Early diagnosis is essential to prevent serious complications. Therefore, we attempted to find highly sensitive and specific detection methods. A comparative evaluation of the detection of cryptococcosis was done by conventional (direct microscopy and culture) and rapid diagnostic [latex agglutination test (LAT), enzyme immunoassay (EIA) and PCR] methods. The study was done on 359 samples from 52 positive patients and 30 negative controls in an Indian set-up. Evaluation was done for cerebrospinal fluid (CSF), serum and urine separately. The diagnostic value of the tests was assessed in pre-treatment samples, and follow-up tests were also done on samples obtained after initiation of treatment. PCR had the highest sensitivity, followed by EIA and LAT, both before and after treatment. The positive detection by LAT, EIA and PCR was the longest in CSF (> 90 days), followed by serum (similar to 65 days) then urine (similar to 45 days) after initiation of treatment. Our results indicated that the sensitivity and specificity of PCR and EIA were comparable in urine, CSF and serum for diagnosis of cryptococcosis.
引用
收藏
页码:1098 / 1105
页数:8
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