Genotyping of Tunisian hepatitis B virus isolates based on the sequencing of preS2 and S regions

被引：38

作者：

Borchani-Chabchoub, I ^{[1
]}

Gargouri, A ^{[1
]}

Mokdad-Gargouri, R ^{[1
]}

机构：

[1] Ctr Biotechnol Sfax, Sfax 3038, Tunisia

来源：

MICROBES AND INFECTION | 2000年 / 2卷 / 06期

关键词：

hepatitis B virus; DNA sequence; genotypes;

D O I：

10.1016/S1286-4579(00)00365-8

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The S nucleotide sequences of five hepatitis B virus strains isolated from plasma samples of Tunisian patients with chronic hepatitis B were determined; the preS2 region of three of them were sequenced. According to the comparative analysis of S peptide sequences with the reported sequences in the database bank, the five hepatitis B strains were shown to be related to the D genotypic group, subtype ayw. The nature of residues at positions 125 and 127 allowed us to distinguish between each subtype of the D group and to class all five Tunisian sequences in the 'ayw2' subtype. Moreover, two of them (1366 and 523) contained a substitution of the invariant Cys69 by Arg and Cys221 by Phe, respectively. Potential structural modifications due to the Cys-Arg change are discussed. (C) 2000 Editions scientifiques et medicales Elsevier SAS.

引用

页码：607 / 612

页数：6

共 16 条

[1]

[Anonymous], TUNISIE MAGHREB MED

[2] SUBTYPE AYW VARIANT OF HEPATITIS-B VIRUS - DNA PRIMARY STRUCTURE-ANALYSIS [J].