Functionalized nucleoside 5′-triphosphates for in vitro selection of new catalytic ribonucleic acids

被引：33

作者：

Matulic-Adamic, J ^{[1
]}

Daniher, AT ^{[1
]}

Karpeisky, A ^{[1
]}

Haeberli, P ^{[1
]}

Sweedler, D ^{[1
]}

Beigelman, L ^{[1
]}

机构：

[1] Ribozyme Pharmaceut Inc, Dept Chem & Biochem, Boulder, CO 80301 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2000年 / 10卷 / 11期

关键词：

D O I：

10.1016/S0960-894X(00)00226-2

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A series of novel 2'-modified nucleoside 5'-triphosphates was synthesized. The amino, imidazole, and carboxylate functionalities were attached to the 5-position of pyrimidine base of these molecules through alkynyl and alkyl spacers, respectively. Two different phosphorylation methods were used to optimize the yields of these highly modified triphosphates. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：1299 / 1302

页数：4

共 16 条

[1] CERIUM(IV)-MEDIATED HALOGENATION AT C-5 OF URACIL DERIVATIVES [J].

ASAKURA, J ;

ROBINS, MJ .

JOURNAL OF ORGANIC CHEMISTRY, 1990, 55 (16) :4928-4933

[2]

AURUP H, 1992, BIOCHEMISTRY-US, V31, P9637

[3] MALDI and FAB mass spectrometry of nucleoside triphosphates: A comparative study [J].

Burgess, K ;

Russell, DH ;

Shitangkoon, A ;

Zhang, AJ .

NUCLEOSIDES & NUCLEOTIDES, 1996, 15 (11-12) :1719-1723

[4]

DEWEY TM, 1995, J AM CHEM SOC, V32, P8475

[5]

DYER RL, 1991, NUCLEIC ACID CHEM, P79

[6] The joys of in vitro selection: chemically dressing oligonucleotides to satiate protein targets [J].

Eaton, BE .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1997, 1 (01) :10-16

[7] RIBONUCLEOSIDES AND RNA [J].

EATON, BE ;

PIEKEN, WA .

ANNUAL REVIEW OF BIOCHEMISTRY, 1995, 64 :837-863

[8] PALLADIUM-CATALYZED SYNTHESIS OF ALKYNYLAMINO NUCLEOSIDES - A UNIVERSAL LINKER FOR NUCLEIC-ACIDS [J].

HOBBS, FW .

JOURNAL OF ORGANIC CHEMISTRY, 1989, 54 (14) :3420-3422

[9] SIMPLE SYNTHESIS OF 5-VINYL- AND 5-ETHYNYL-2'-DEOXYURIDINE-5'-TRIPHOSPHATES [J].

KOVACS, T ;

OTVOS, L .

TETRAHEDRON LETTERS, 1988, 29 (36) :4525-4528

[10] RAPID AND EFFICIENT SYNTHESIS OF NUCLEOSIDE 5'-O-(1-THIOTRIPHOSPHATES), 5'-TRIPHOSPHATES AND 2',3'-CYCLOPHOSPHOROTHIOATES USING 2-CHLORO-4H-1,3,2-BENZODIOXAPHOSPHORIN-4-ONE [J].

LUDWIG, J ;

ECKSTEIN, F .

JOURNAL OF ORGANIC CHEMISTRY, 1989, 54 (03) :631-635

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