Selective cholinergic depletion of the hippocampus spares both behaviorally induced arc transcription and spatial learning and memory

被引:32
作者
Fletcher, Bonnie R.
Baxter, Mark G.
Guzowski, John F.
Shapiro, Matthew L.
Rapp, Peter R.
机构
[1] CUNY Mt Sinai Sch Med, Fishberg Dept Neurosci, New York, NY 10029 USA
[2] CUNY Mt Sinai Sch Med, Alfred B & Gudrun J Kastor Neurobiol Aging Labs, New York, NY 10029 USA
[3] Univ Oxford, Dept Expt Psychol, Oxford OX1 3UD, England
[4] Univ Calif Irvine, Dept Neurobiol & Behav, Irvine, CA USA
关键词
acetylcholine; medial septum; immediate early gene; saporin;
D O I
10.1002/hipo.20261
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We demonstrated previously that when hippocampal-dependent learning and plasticity are compromised by fornix lesions, behaviorally induced expression of the immediate early gene, Arc, is correspondingly low. The medial septum and the vertical diagonal band are major sources of subcortical afferents that innervate the hippocampus via the fornix. Here we assessed the specific contribution of cholinergic afferents from these regions to the impairments in spatial learning and behavioral induction of Arc transcription produced by fornix lesions. The immunotoxin, 192 IgG-saporin, was used to produce selective lesions of cholinergic cell bodies in the medial septum and vertical diagonal band. Rats were then trained on both cued and spatial delayed match-to-place tasks in a radial arm water maze. Animals with 192 IgG-saporin lesions learned both cue and place discrimination tasks in the water maze normally, and showed only a mild and transient impairment when switching from the cued to the spatial version of the task. Following behavioral testing, rats explored two novel environments sequentially in a setting known to induce Arc expression in hippocampal pyramidal neurons. In marked contrast to the effects of complete fornix transection, quantitative in situ autoradiography revealed no differences in Arc mRNA expression between sham and lesion animals in CA1, CA3 or stratum radiatum. The conclusion from these data is that cholinergic deafferentation alone cannot account for the spatial learning deficits or impaired behavioral induction of Arc transcription produced by fornix lesions. (c) 2007 Wiley-Liss, Inc.
引用
收藏
页码:227 / 234
页数:8
相关论文
共 61 条
[1]   AN ANALYSIS OF THE ORIGINS OF THE CHOLINERGIC AND NONCHOLINERGIC SEPTAL PROJECTIONS TO THE HIPPOCAMPAL-FORMATION OF THE RAT [J].
AMARAL, DG ;
KURZ, J .
JOURNAL OF COMPARATIVE NEUROLOGY, 1985, 240 (01) :37-59
[2]  
Baxter MG, 2001, METH MOL B, V166, P249
[3]  
Baxter MG, 1997, J NEUROSCI, V17, P5230
[4]  
BIERER LM, 1995, J NEUROCHEM, V64, P749
[5]   Effects of hippocampal cholinergic deafferentation on learning strategy selection in a visible platform version of the water maze [J].
Bizon, JL ;
Han, JS ;
Hudon, C ;
Gallagher, M .
HIPPOCAMPUS, 2003, 13 (06) :676-684
[6]   Anatomical, electrophysiological and pharmacological studies of ascending brainstem hippocampal synchronizing pathways [J].
Bland, BH ;
Oddie, SD .
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS, 1998, 22 (02) :259-273
[7]   Theta oscillations in the hippocampus [J].
Buzsáki, G .
NEURON, 2002, 33 (03) :325-340
[8]   Selective lesions of the nucleus basalis magnocellularis impair cognitive flexibility [J].
Cabrera, SM ;
Chavez, CM ;
Corley, SR ;
Kitto, MR ;
Butt, AE .
BEHAVIORAL NEUROSCIENCE, 2006, 120 (02) :298-306
[9]   Cholinergic and noncholinergic septal neurons modulate strategy selection in spatial learning [J].
Cahill, JFX ;
Baxter, MG .
EUROPEAN JOURNAL OF NEUROSCIENCE, 2001, 14 (11) :1856-1864
[10]   A re-examination of the role of basal forebrain cholinergic neurons in spatial working memory [J].
Chappell, J ;
McMahan, R ;
Chiba, A ;
Gallagher, M .
NEUROPHARMACOLOGY, 1998, 37 (4-5) :481-487