High-throughput method development approaches for bioanalytical mass spectrometry

被引：13

作者：

Janiszewski, JS ^{[1
]}

Swyden, MC ^{[1
]}

Fouda, HG ^{[1
]}

机构：

[1] Pfizer Inc, Div Cent Res, Groton, CT 06340 USA

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2000年 / 38卷 / 06期

关键词：

D O I：

10.1093/chromsci/38.6.255

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rational approach to the development and optimization of solid-phase extraction (SPE) methods is described. The semiautomated scheme allows for the simultaneous testing of multiple chemistries using a custom multiple-sorbent 96-well method development plate. Optimized extraction conditions for up to five analytes are determined in a single 2.5-h experiment. The experiment can be tailored to determine SPE conditions (including wash protocols) for related analytes. Data obtained by liquid chromatography-atmospheric pressure ionization-mass spectrometry allows the quantitation of absolute recovery and selection of the best extraction conditions for approximately 100 analytes of diverse structure. Optimized extraction protocols yielding at least 80% recovery are determined for 81% of the analytes. For 96% of the analytes screened, extraction conditions resulting in recoveries of ≥ 60% are determined. The most generic set of SPE conditions consist of either C8 or C18 sorbent with an eluent composition of acetonitrile with 5mM nitric acid added.

引用

页码：255 / 258

页数：4