A large-scale collection of phenotypic data describing an insertional mutant population to facilitate functional analysis of rice genes

被引：111

作者：

Miyao, Akio

Iwasaki, Yukimoto

Kitano, Hidemi

Itoh, Jun-Ichi

Maekawa, Masahiko

Murata, Kazumasa

Yatou, Osamu

Nagato, Yasuo

Hirochika, Hirohiko

机构：

[1] Natl Inst Agrobiol Sci, Div Genome & Biodivers Res, Tsukuba, Ibaraki 3058602, Japan

[2] Fukui Prefectural Univ, Fac Biosci, Fukui 9101195, Japan

[3] Nagoya Univ, Biosci & Biotechnol Ctr, Nagoya, Aichi 4648601, Japan

[4] Univ Tokyo, Grad Sch Agr & Life Sci, Tokyo 1138657, Japan

来源：

PLANT MOLECULAR BIOLOGY | 2007年 / 63卷 / 05期

关键词：

Oryza sativa; insertion mutagenesis; phenotyping; retrotransposon; database;

D O I：

10.1007/s11103-006-9118-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to facilitate the functional analysis of rice genes, we produced about 50,000 insertion lines with the endogenous retrotransposon Tos17. Phenotypes of these lines in the M-2 generation were observed in the field and characterized based on 53 phenotype descriptors. Nearly half of the lines showed more than one mutant phenotype. The most frequently observed phenotype was low fertility, followed by dwarfism. Phenotype data with photographs of each line are stored in the Tos17 mutant panel web-based database with a dataset of sequences flanking Tos17 insertion points in the rice genome (http://tos.nias.affrc.go.jp/). This combination of phenotypic and flanking sequence data will stimulate the functional analysis of rice genes.

引用

页码：625 / 635

页数：11

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