Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators

被引：1488

作者：

Tian, Lin ^{[1
]}

Hires, S. Andrew ^{[1
]}

Mao, Tianyi ^{[1
]}

Huber, Daniel ^{[1
]}

Chiappe, M. Eugenia ^{[1
]}

Chalasani, Sreekanth H. ^{[2
]}

Petreanu, Leopoldo ^{[1
]}

Akerboom, Jasper ^{[1
]}

McKinney, Sean A. ^{[1
]}

Schreiter, Eric R. ^{[3
]}

Bargmann, Cornelia I. ^{[2
]}

Jayaraman, Vivek ^{[1
]}

Svoboda, Karel ^{[1
]}

Looger, Loren L. ^{[1
]}

机构：

[1] Howard Hughes Med Inst, Ashburn, VA USA

[2] Rockefeller Univ, Howard Hughes Med Inst, Lab Neural Circuits & Behav, New York, NY 10021 USA

[3] Univ Puerto Rico, Dept Chem, San Juan, PR 00936 USA

来源：

NATURE METHODS | 2009年 / 6卷 / 12期

关键词：

GREEN FLUORESCENT PROTEINS; GENETICALLY ENCODED INDICATORS; IN-VIVO; CA2+ INDICATORS; PYRAMIDAL NEURONS; BARREL CORTEX; TROPONIN-C; RESOLUTION; PROJECTIONS; CALMODULIN;

D O I：

10.1038/nmeth.1398

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Genetically encoded calcium indicators (GECIs) can be used to image activity in defined neuronal populations. However, current GECIs produce inferior signals compared to synthetic indicators and recording electrodes, precluding detection of low firing rates. We developed a single-wavelength GCaMP2-based GECI (GCaMP3), with increased baseline fluorescence (3-fold), increased dynamic range (3-fold) and higher affinity for calcium (1.3-fold). We detected GCaMP3 fluorescence changes triggered by single action potentials in pyramidal cell dendrites, with signal-to-noise ratio and photostability substantially better than those of GCaMP2, D3cpVenus and TN-XXL. In Caenorhabditis elegans chemosensory neurons and the Drosophila melanogaster antennal lobe, sensory stimulation-evoked fluorescence responses were significantly enhanced with GCaMP3 (4-6-fold). In somatosensory and motor cortical neurons in the intact mouse, GCaMP3 detected calcium transients with amplitudes linearly dependent on action potential number. Long-term imaging in the motor cortex of behaving mice revealed large fluorescence changes in imaged neurons over months.

引用

页码：875 / U113

页数：10

共 45 条

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Crystal Structures of the GCaMP Calcium Sensor Reveal the Mechanism of Fluorescence Signal Change and Aid Rational Design [J].